Brucella Antibody Rapid Test Uncut Sheet
The Brucella Antibody Rapid Test is a chromatographic immunoassay (CIA) for direct qualitative detection of Brucella antibody in mammal and human serum or plasma. This assay provides only a preliminary result and all positive specimens should be confirmed with other qualified assays.
Product Overview
The Brucella Antibody Rapid Test is a chromatographic immunoassay (CIA) for direct qualitative detection of Brucella antibody in mammal and human serum or plasma. This assay provides only a preliminary result and all positive specimens should be confirmed with other qualified assays.
Product Parameters
| Parameter | Specification |
|---|---|
| Code | FUBR9 |
| Specimen | Mammal and human serum or plasma |
| Format | Rapid Test Uncut Sheet |
Summary and Explanation
Brucella is strictly aerobic, Gram-negative coccobacillus which causes Brucellosis. This organism is sometimes carried by animals and only causes incidental infections in humans. The four species of this genus that can infect humans are named for the animal which they are most commonly found: B. abortus (cattle), B. suis (swine), B. melitensis (goats), B. canis (dogs). The cattle and dairy industries seem to be the primary source of infection in the United States. Brucella can enter the body via the skin, respiratory tract, or digestive tract. Once there, this intracellular organism can enter the blood and the lymphatics where it multiplies inside phagocytes and eventually cause bacteremia (bacterial blood infiltration). Symptoms vary from patient to patient but can include high fever, chills, and sweating. Afflicted individuals are usually treated with streptomycin or erythromycin.
Test Procedure
Allow the test cassette, specimen, buffer, and/or controls to reach room temperature (15-30"C) prior to testing.
1.Bring the pouch to room temperature before opening. Remove the test cassette from the
sealed pouch and use it within one hour.
2.Place the test cassette on a clean and level surface. Hold the dropper vertically, draw the specimen and transfer the specimen to the sample well of the test cassette (two drops/approximately 50ul) to the sample well and start the timer. Avoid trapping air bubbles in the sample well.
3. Wait for the colored line(s)to appear. The test result should be read at 10 minutes. Do not interpret the result after 20 minutes.
Result Interpretation
Negative result: if there is only a quality control line C, the detection line is colorless, indicating that Brucella antibody has not been detected and the result is negative.
Positive result: if both the quality control line C and the detection line appear, Brucella antibody has been detected and the result is positive.
Invalid result: if the quality control line C is not observed, it will be invalid regardless of whether there is detection line, and the test shall be conducted again.