Classical Swine Fever (CSFV) Antibody & Antigen
Classical Swine Fever (CSFV) antibody and antigen raw materials for immunoassay development, reagent screening and rapid-test optimization. Source: Mouse (Antibody) / E. coli (Antigen); application: Classical Swine Fever.
Product Parameters
| Parameter | Specification |
|---|---|
| Code | FBVP3/FACS9 |
| Source | Mouse (Antibody) / E. coli (Antigen) |
| Detection Method | ELISA; ICA |
Background
Swine fever, commonly known as "bad intestine disease", is a highly infectious disease, which is one of the main infectious diseases threatening the pig industry. Chronic necrotizing enteritis of cellulose, often secondary to paratyphoid and pasteurellosis.
Clinical Significance
1.Detection of virus antigen the rapid detection of virus antigen of CSFV is very important for the diagnosis of CSFV. At present, the rapid and sensitive detection method is to detect tonsil tissue by immunohistochemistry. CSFV antigen in tonsils can be detected as early as 2d after infection. Lymph nodes, spleen and pancreas can be used as tissue samples for early detection of the virus. Delas Mulas and Narita et al. reported an immunohistochemical method for the detection of CSFV antigen. That is to say, immunohistochemistry of tissue samples fixed in formalin and embedded in paraffin was detected by monoclonal antibody against E2 glycoprotein. The advantage of this method is that it can detect the samples stored for a long time. And formalin fixed tissue can no longer be isolated virus. Immunohistochemical method using linase-biotin-peroxidase (ABc) can also be used for the detection of antigen in tissue sections of swine fever virus. It is reported that flow cytometry can also be used to detect CSFV in blood samples, but its sensitivity is low. The virus of piglets infected with swine fever was detected by antigen capture ELISA. But its sensitivity and specificity are lower than traditional laboratory methods.
2.The isolation of infectious virus is the most specific method to detect infectious swine fever virus in vitro. Several methods have been reported to isolate viruses from tissues and whole blood. Some researchers, however, have found that white blood cells and monocytes take longer to catch the virus after an animal is infected. Therefore, the early diagnosis of swine fever. Whole blood or plasma may be more sensitive than white blood cells. The cells most commonly used for isolation of CSFV are porcine kidney cells (PKl 5 or SK6) or porcine testicular cells (sT). A porcine kidney - derived cell line (fs-l3) was established in Japan. Fs-l3 cells are large domed in the absence of virus infection. Several times to tens of times more than ordinary cells. However, once infected with CSFV, the large round spheres of fs-l3 cells disappeared. This characteristic has been used to determine the neutralization test of swine fever virus and other biological characteristics. It has been found that the cause of the cytopathic strain is a genetic change. From the first nucleotide at the 5 'end to 4764 nucleotides were missing. Genes including the 5 '-terminal non-coding region, autoproteolytic enzymes, capsid proteins, glycoproteins (E0, E1, E2), nonstructural proteins ns-1, and ns-2 are all missing.